LOCUS       283_YPET_C1_SEC_       10479 bp ds-DNA     circular     01-JUN-2015
DEFINITION  Cloning vector pUC19, complete sequence.
KEYWORDS    .
SOURCE      Cloning vector pUC19
  ORGANISM  Cloning vector pUC19 other sequences; artificial sequences;
            vectors.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   New England Biolabs.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2007) Research Department, New England Biolabs,
            240 County Road, Ipswich, MA 01938, USA
COMMENT     To prepare vector for cloning: 
COMMENT     -Digest with ClaI and SpeI to fuse 5' homology arm directly to mNG,
            or 
COMMENT     -Digest with AvrII and SpeI to include a flexible linker (for
            C-terminal tags).
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    404..1010
                     /label=This sequence will be replaced by the homology arm
                     /ApEinfo_fwdcolor=#cc9966
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             join(2770..2910,2958..3791)
                     /label=SQT-1
                     /ApEinfo_fwdcolor=#00cccc
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             6100..7125
                     /note="hygromycinR"
                     /label=hygR
                     /ApEinfo_fwdcolor=#006666
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1986..2015
                     /label=myc
                     /ApEinfo_fwdcolor=#6666ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_RNA        complement(8660..9212)
                     /note="RNAII transcript (complementary strand)"
                     /label=RNAII transcript (complementary strand)
                     /ApEinfo_fwdcolor=#cccc33
                     /ApEinfo_revcolor=#999933
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_RNA        9102..9209
                     /note="RNAI transcript"
                     /label=RNAI transcript
                     /ApEinfo_fwdcolor=#cccc33
                     /ApEinfo_revcolor=#999933
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(join(4164..4405,4457..4827,4879..5294))
                     /label=Cre
                     /ApEinfo_fwdcolor=#6699cc
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(8660..9248)
                     /note="pMB1 origin of replication (counter-clockwise)
                     (RNAII -35 to RNA/DNA switch point)"
                     /label=pMB1 origin of replication (counter-clockwise) (RN
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -35_signal      9066..9071
                     /note="RNAI promoter (clockwise); TTGAAG"
                     /label=RNAI promoter (clockwise); TTGAAG
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -35_signal      complement(9243..9248)
                     /note="RNAII promoter (counter-clockwise); TTGAGA"
                     /label=RNAII promoter (counter-clockwise); TTGAGA
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     intron          1932..1942
                     /label=intron
                     /ApEinfo_fwdcolor=#cc66ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -10_signal      9088..9093
                     /note="RNAI promoter (clockwise); GCTACA"
                     /label=RNAI promoter (clockwise); GCTACA
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1943..1976
                     /label=loxP
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     -10_signal      complement(9222..9227)
                     /note="RNAII promoter (counter-clockwise); CGTAAT"
                     /label=RNAII promoter (counter-clockwise); CGTAAT
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(9422..10081)
                     /label=AmpR
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1983..1985
                     /label=spacer
                     /ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_difference 3021..3021
                     /label=e1350
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(9419..10279)
                     /gene="bla"
                     /note="ampR (confers resistance to ampicillin)"
                     /codon_start=1
                     /product="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label=beta-lactamase
                     /ApEinfo_fwdcolor=#00cccc
                     /ApEinfo_revcolor=#006666
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      2019..2399
                     /label=let-858 Terminator
                     /ApEinfo_fwdcolor=#cc0000
                     /ApEinfo_revcolor=#cc0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     intron          1977..1982
                     /label=intron(1)
                     /ApEinfo_label=intron
                     /ApEinfo_fwdcolor=#cc66ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     complement(8254..8274)
                     /label=M13-rev
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     5'UTR           join(2684..2711,2768..2769)
                     /label=sqt-1 5'UTR
                     /ApEinfo_fwdcolor=#ccffcc
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             join(1038..1340,1392..1466,1518..1637,1689..1904)
                     /label=mYPET
                     /ApEinfo_fwdcolor=#cccc00
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     1905..1925
                     /label=TEV site
                     /ApEinfo_fwdcolor=#ff6600
                     /ApEinfo_revcolor=#ff6600
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    661..966
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
                     /label=ccdB
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     rep_origin      complement(8642..9324)
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     3'UTR           3792..3828
                     /label=sqt-1 3'UTR
                     /ApEinfo_fwdcolor=#cc3333
                     /ApEinfo_revcolor=#cc3333
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        complement(5295..5694)
                     /label=hsp16-41 promoter
                     /ApEinfo_fwdcolor=#0080c0
                     /ApEinfo_revcolor=#0080c0
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    7609..7635
                     /label=Flag
                     /ApEinfo_fwdcolor=#ff6699
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        2400..2769
                     /label=Psqt1
                     /ApEinfo_fwdcolor=#33cc00
                     /ApEinfo_revcolor=#33cc00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             754..963
                     /label=ccdB(1)
                     /ApEinfo_label=ccdB
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    7582..7608
                     /label=Flag(1)
                     /ApEinfo_label=Flag
                     /ApEinfo_fwdcolor=#ff3366
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7543..7554
                     /label=spacer(1)
                     /ApEinfo_label=spacer
                     /ApEinfo_fwdcolor=#cccccc
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     protein_bind    7555..7581
                     /label=Flag(2)
                     /ApEinfo_label=Flag
                     /ApEinfo_fwdcolor=#cc3366
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     3'UTR           complement(3829..4160)
                     /label=tbb-2 3'UTR
                     /ApEinfo_fwdcolor=#cc3333
                     /ApEinfo_revcolor=#993333
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     intron          7537..7542
                     /label=intron(2)
                     /ApEinfo_label=intron
                     /ApEinfo_fwdcolor=#cc66ff
                     /ApEinfo_revcolor=#ff00ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     3'UTR           7151..7502
                     /label=unc-54 3'UTR
                     /ApEinfo_fwdcolor=#990000
                     /ApEinfo_revcolor=#cc0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7503..7536
                     /label=loxP(1)
                     /ApEinfo_label=loxP
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        5695..6057
                     /note="Prps-0"
                     /note="/ApEinfo_fwdcolor=pink"
                     /note="/ApEinfo_revcolor=pink; /ApEinfo_fwdcolor=pink;
                     /ApEinfo_revcolor=pink; /ApEinfo_graphicformat=arrow_data
                     {{0 1 2 0 0 -1} {} 0} width 5 offset 0"
                     /label=Prps-0
                     /ApEinfo_fwdcolor=#339933
                     /ApEinfo_revcolor=#339933
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8243..8249
                     /label=This sequence will be deleted if ClaI is used
                     /ApEinfo_fwdcolor=#ffcc99
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7636..8242
                     /label=This sequence will be replaced by the homology
                     arm(1)
                     /ApEinfo_label=This sequence will be replaced by the
                     homology arm
                     /ApEinfo_fwdcolor=#cc9966
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(7680..7985)
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
                     /label=ccdB(2)
                     /ApEinfo_label=ccdB
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(7683..7892)
                     /label=ccdB(3)
                     /ApEinfo_label=ccdB
                     /ApEinfo_fwdcolor=yellow
                     /ApEinfo_revcolor=yellow
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_structure  1011..1037
                     /label=Flexible Linker (will be deleted if ClaI is used)
                     /ApEinfo_fwdcolor=#999999
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     378..395
                     /label=M13-fwd
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtcgccg gcactagtTA CCCGAAGTAT
      421 GTCAAAAAGA GGTATGCTAT GAAGCAGCGT ATTACAGTGA CAGTTGACAG CGACAGCTAT
      481 CAGTTGCTCA AGGCATATAT GATGTCAATA TCTCCGGTTC GGTAAGCACA ACCATGCAGA
      541 ATGAAGCCCG TCGTCTGCGT GCCGAACGCT GGAAAGCGGA AAATCAGGAA GGGATGGCTG
      601 AGGTCGCCCG GTTTATTGAA ATGAACGGCT CTTTTGCCGA CGAGAACAGG GGCTGGTGAA
      661 ATGCAGTTTA AGGTTTACAC CTATAAAAGA GAGAGCCGTT ATCGTCTGTT TGTGGATGTA
      721 CAGAGTGATA TTATTGACAC GCCCGGGCGA CGGATGGTGA TCCCCCTGGC CAGTGCACGT
      781 CTGCTGTCAG ATAAAGTCCC CCGTGAACTT TACCCGGTGG TGCATATCGG GGATGAAAGC
      841 TGGCGCATGA TGACCACCGA TATGGCCAGT GTGCCGGTCT CCGTTATCGG GGAAGAAGTG
      901 GCTGATCTCA GCCACCGCGA AAATGACATC AAAAACGCCA TTAACCTGAT GTTCTGGGGA
      961 ATATAAATGT CAGGCTCCCT TATACACAGC CAGTCTGCAG GTCGAcctag GGAGCATCGG
     1021 GAGCCTCAGG AGCATCGATG TCAAAAGGAG AAGAGTTATT TACAGGAGTT GTACCTATCC
     1081 TCGTCGAGCT CGACGGAGAC GTCAACGGAC ACAAGTTCTC CGTCTCCGGA GAGGGAGAGG
     1141 GAGACGCCAC CTACGGAAAG CTCACCCTCA AGCTCCTCTG CACCACCGGA AAGCTCCCAG
     1201 TCCCATGGCC AACCCTCGTC ACCACCCTCG GATACGGAGT CCAATGCTTC GCCCGTTACC
     1261 CAGACCACAT GAAGCAACAC GACTTCTTCA AGTCCGCCAT GCCAGAGGGA TACGTCCAAG
     1321 AGCGTACCAT CTTCTTCAAG gtaagtttaa acatatatat actaactaac cctgattatt
     1381 taaattttca gGACGACGGA AACTACAAGA CCCGTGCCGA GGTCAAGTTC GAGGGAGACA
     1441 CCCTCGTCAA CCGTATCGAG CTCAAGgtaa gtttaaacag ttcggtacta actaaccata
     1501 catatttaaa ttttcagGGA ATCGACTTCA AGGAGGACGG AAACATCCTC GGACACAAGC
     1561 TTGAGTACAA CTACAACTCC CACAACGTCT ACATCACCGC CGACAAGCAA AAGAACGGAA
     1621 TCAAGGCCAA CTTCAAGgta agtttaaaca tgattttact aactaactaa tctgatttaa
     1681 attttcagAT CCGTCACAAC ATCGAGGACG GAGGAGTCCA ACTCGCCGAC CACTACCAAC
     1741 AAAACACCCC AATCGGAGAC GGACCAGTCC TCCTCCCAGA CAACCACTAC CTCTCCTACC
     1801 AATCCAAGCT CTTCAAGGAC CCAAACGAGA AGCGTGACCA CATGGTCCTC CTTGAGTTCC
     1861 TCACCGCCGC CGGAATCACC GAGGGAATGA ACGAGCTCTA CAAGGAGAAT CTGTACTTTC
     1921 AATCCGGAAA Ggtaagttta aaATAACTTC GTATAGCATA CATTATACGA AGTTATtttc
     1981 agGGAGAGCA AAAGCTTATC TCCGAGGAGG ACCTCTAAGG ATGATCGACG CCaACGTCGT
     2041 TGAATTTTCA AATTTTAAAT ACTGAATATT TGTTTTTTTT CCTATTATTT ATTTATTCTC
     2101 TTTGTGTTTT TTTTCTTGCT TTCTAAAAAA TTAATTCAAT CCAAATCTAA acattttttt
     2161 ttctctttcc gtctcccaat tcgtattccg ctcctctcat ctgaacacaa tgtgcaagtt
     2221 tatttatctt ctcgctttca tttcattagg acgtgggggg aattggtgga agggggaaac
     2281 acacaaaagg atgatggaaa tgaaataagg acacacaata tgcaacaaca ttcaattcag
     2341 aaatatggag gaaggtttaa aagaaaacat aaaaatatat agaggaggaa ggaaactagc
     2401 tgccctcgat tgtctatctt ccattctcac caagtttcaa caactttatt cagtcattct
     2461 tctctggttc atttcccaac caatcttccc gaataaagcc catctgtcct ttcggccaca
     2521 ttcctcacct acatccgtac tccttatctc ccgttcttat ccagtctttt atcgtctctg
     2581 cgtctcgcac actgtctcgt cggtaatgta aaaaaaaaac aaaaaaaact tgaaaacttg
     2641 cggaaagatt tcctaataaa aggagacgaa tccggtagga aatcgacaag tctctgcttc
     2701 tcgaagacta ggtgagttac atttctttgg ccgaattatt ttgaaatcct aaatttgata
     2761 atttcagagA TGTCTGTAAA ACTTGCGTGT TATGTGACGG CTTCGGTCAC TGTCGCCACT
     2821 CTTATGGTAT GCTTCATGAC CATGTCAACC ATCTACTCGG AGGTTGATGG ATTCAGAGAG
     2881 AAGCTTGATA CCGAGATGAA TGTGTTCAGA gtgagttaaa ttatattttg aattttaaat
     2941 aatttttaat tttctagCAA TCTACCAATG GATTGTGGAA GGACATAGTT GTCATCGGAA
     3001 GATCTAGCAA GCGTGTCCGT TGTCAATATG AAGAGACCAA CGCTACCCCA ACTCCACATG
     3061 CTGATGGATC CCCATCTGCT CCACCAGGTC AACCACCAGC AGTTCCACCA GTCTTCAACC
     3121 AGCCAAAGAC TCCAAATGGA GCCAATGGAA ATGGACCAAC CTGCAACTGC AATGCTGATA
     3181 ACAAGTGCCC AGCTGGACCA TCCGGACCAA AGGGAGTTCC AGGAGTTCCA GGACTCGACG
     3241 GAGTTCCAGG ACTTGACGGT GTTCCAGGAG TTGGAGCTGA TGATATCGCT CCACAACGCG
     3301 AGTCTGTCGG ATGCTTCACT TGCCCACAAG GACCAGTTGG ACCACCAGGA GCTCTTGGAA
     3361 GACCAGGACC ACGTGGACTT CCAGGACCAA GAGGACAAAA TGGAAACCCA GGAAGAGATG
     3421 GACAACCAGG ACATCCAGGA GAGCAAGGAT CATCCGGTCA AATCGGAAAG ATCGGAGAGC
     3481 CAGGACCACC AGGAGAGAAG GGACGCGACG CCGAGCATCC AATCGGAAGA CCAGGACCAA
     3541 AGGGACCAAG AGGAGATCAA GGACCAACAG GACCAGCTGG ACAGAACGGT CTTCACGGAC
     3601 CACCAGGAGA GCCAGGAACC GTTGGACCAG AAGGACCATC TGGAAAGCAA GGACGTCAAG
     3661 GACCAGACGG AACCCAGGGA GAGACTGGAC CAGACGGAAG ACCAGGAAAG GATGCCGAGT
     3721 ACTGCCAGTG CCCAGACAAG TCTCCACCAT CAGAGGCTGT CAACGCCAAC CGTGGATACA
     3781 GAAATATCTA Aattgttggt gttttctaat aaaatatttg agattcagtg agactttttt
     3841 cttggcggca caataaagtt tcctcttttt gttgggtcac tcaacgaaca caatacaaat
     3901 atttgagcgc tcgcattttt ctcaaccttt cgggtttaaa gcacttcaaa atggaattga
     3961 agagagattg tgaaagtaca tcagacagaa tcacattttg atagcattca cttcactcag
     4021 atgcaaactt ttcagtgact ataaaaagtc ataaaataat aacattaaaa aagcaaataa
     4081 attagcgaga gaattttttg acaaaaagaa agaagagtga tagagaagaa gggaatgctt
     4141 gaaaggattt tgcatttatc ttaATCACCG TCTTCGAGAA GACGAACCAT GGCGCCAGTT
     4201 TCAGAATCCA AGTTTCTAAT ATAGTTCATC ACAATGTTGA CGTTGGTCCA ACCTCCGGCT
     4261 TGCATAATCT CTGGGATGGA AACTCCAGCG CGAGCCATGT CACGGGCAGC TCCGACACGG
     4321 GCGGAATGTC CAGACCAAGC CAAGTAACGT TGTCCGGAAT CATCCTTGGC TCCGTAGATG
     4381 AGACGGTGGG TAGCTTCGAA GATTCctgaa aatttatttt cagcttattc ttagctaata
     4441 tgttgtttaa acttacCTTC AAGAGCTCGA GTCGAGAGTT GTGATGTTGC ACTCGGGGCA
     4501 GCAACTCCGT TCTTGCGAAC ACGACAGAAC AGGTAGTTGT TTGGATCATC GGCGACTCCG
     4561 GAAACCGAAA TCCAGCGCTC AACGAGCTTG GTAACTCCAA GGGAGAGCGC TTTTTCGACT
     4621 CCGGCAGTCG AGACCAGCGT TTTTGTACGT CCGATGTGGA TGAGCATTCT TCCTCCGTCT
     4681 GTGCGACTAA TATCCTTGAC GCGAATGCGG GCAATTTCGG CGATACGGAG AAGTGTGTTG
     4741 TAAGCAATTC CGAGGAAGGC GAGGTTTCGG ATGTCCTGGC AACGATCGGA ATTTTCCATA
     4801 AGAGAACGGA CCTGATCGAA GTCGGTTctg aaaatttcgt caagtcttag gattgaatac
     4861 attaatattt aaacttacCT TTCGAAGGCG AGGGCTTGCT TGGCTCTCTC TCCAGCATCA
     4921 ACATTTTCCT TGCGGATACG TCTCATTACG AGAGATACGG CATTAGAATC TGATGGGCGT
     4981 GGAAGTCCAG ATCGGCGATG GAGCATATTG AGCTGTCCGA GATGTTGTTG GATCGTCTTC
     5041 ACCGCCAATC CACGAGCTTG GAGGTAGAGC AAGTAGTCAC GAACGTCTTC TGGCTCGGCT
     5101 GGGAACCACT TGCGGTTGTT AAGCTTGCAC CAGGCAGCCC AAGAACGGCA CACTGAGAGA
     5161 AGCATCTTCC AGGTGTGCTC GGAGAAGGCT TGTCTGTCAC GGAACATATC CATGAGGTTC
     5221 TTGCGAACTT CATCACTTGT CGCGTCAACT GGAAGTGCTG GAAGATTTTG ATGAACAGTG
     5281 AGAAGATTTG ACATattttc gaagtttttt agatgcacta gaacaaagcg tgttggcttc
     5341 ctctgagccc gctttcctta tatacccgca ttctgcagcc ttacagaatg ttctagaagg
     5401 tcctagatgc attcgtttga aaatactccc ggtgggtgca aagagacgca gacggaaaat
     5461 gtatctgggt ctctttattg tgtacactac ttttccatgt accgaatgtg agtcgccctc
     5521 cttttgcaac aagcagctcg aatgttctag aaaaaggtgg aaaatagtat aaataccgtt
     5581 gaaaataaat accgaacaac atttgctcta attgtgaaat tagaaatctt caaactataa
     5641 tcatgtcact ttaccactat ttccgtccag ctcaacgttc cgtttttggt gatcATTTTT
     5701 GCTTTCGTCG TAAATCTACA CACGCGTCTC TTCCGTGCGA GAGTCCAAGC CAGCAGCCAA
     5761 ATTCGTTGAC TGAGTATTCA ACGTTTATAC GTTGTCGGCA ACGAGAAATA GGAAAATGCA
     5821 TCGGGAAATG TTCTTTTTTC GATTTTTTCC AAGGTTTTGA CAAATTTTAC CACGAATTTT
     5881 GCTATGTTTT CAATTAAAAA ATATGTTATT CAACTGTTTC TATGAGGAAA ATAAGGCTTT
     5941 GCATGTAATT TTCTTATTCA GCATAATTTT TAATTAATTT GAATTTTCTG TCCTAACGTT
     6001 TATTTTGTTT TCTTGGTTAT GACTGATCTG AAATTAATTT TTGAATTTTA AGGTAATATG
     6061 TCAGGCGGTG CCGCAAGTTT GTACAAAAAA GCAGGCTCCA TGAAAAAGCC TGAACTCACC
     6121 GCGACGTCTG TCGAGAAGTT TCTGATCGAA AAGTTCGACA GCGTCTCCGA CCTGATGCAG
     6181 CTCTCGGAGG GCGAAGAATC TCGTGCTTTC AGCTTCGATG TAGGAGGGCG TGGATATGTC
     6241 CTGCGGGTAA ATAGCTGCGC CGATGGTTTC TACAAAGATC GTTATGTTTA TCGGCACTTT
     6301 GCATCGGCCG CGCTCCCGAT TCCGGAAGTG CTTGACATTG GGGAATTCAG CGAGAGCCTG
     6361 ACCTATTGCA TCTCCCGCCG TGCACAGGGT GTCACGTTGC AAGACCTGCC TGAAACCGAA
     6421 CTGCCCGCTG TTCTGCAGCC GGTCGCGGAG GCCATGGATG CGATCGCTGC GGCCGATCTT
     6481 AGCCAGACGA GCGGGTTCGG CCCATTCGGA CCGCAAGGAA TCGGTCAATA CACTACATGG
     6541 CGTGATTTCA TATGCGCGAT TGCTGATCCC CATGTGTATC ACTGGCAAAC TGTGATGGAC
     6601 GACACCGTCA GTGCGTCCGT CGCGCAGGCT CTCGATGAGC TGATGCTTTG GGCCGAGGAC
     6661 TGCCCCGAAG TCCGGCACCT CGTGCACGCG GATTTCGGCT CCAACAATGT CCTGACGGAC
     6721 AATGGCCGCA TAACAGCGGT CATTGACTGG AGCGAGGCGA TGTTCGGGGA TTCCCAATAC
     6781 GAGGTCGCCA ACATCTTCTT CTGGAGGCCG TGGTTGGCTT GTATGGAGCA GCAGACGCGC
     6841 TACTTCGAGC GGAGGCATCC GGAGCTTGCA GGATCGCCGC GGCTCCGGGC GTATATGCTC
     6901 CGCATTGGTC TTGACCAACT CTATCAGAGC TTGGTTGACG GCAATTTCGA TGATGCAGCT
     6961 TGGGCGCAGG GTCGATGCGA CGCAATCGTC CGATCCGGAG CCGGGACTGT CGGGCGTACA
     7021 CAAATCGCCC GCAGAAGCGC GGCCGTCTGG ACCGATGGCT GTGTAGAAGT ACTCGCCGAT
     7081 AGTGGAAACC GACGCCCCAG CACTCGTCCG AGGGCAAAGG AATAGACCCA GCTTTCTTGT
     7141 ACAAAGTGGG TCCAATTACT CTTCAACATC CCTACATGCT CTTTCTCCCT GTGCTCCCAC
     7201 CCCCTATTTT TGTTATTATC AAAAAACTTC TCTTAATTTC TTTGTTTTTT AGCTTCTTTT
     7261 AAGTCACCTC TAACAATGAA ATTGTGTAGA TTCAAAAATA GAATTAATTC GTAATAAAAA
     7321 GTCGAAAAAA ATTGTGCTCC CTCCCCCCAT TAATAATAAT TCTATCCCAA AATCTACACA
     7381 ATGTTCTGTG TACACTTCTT ATGTTTTTTA CTTCTGATAA ATTTTTTTGA AACATCATAG
     7441 AAAAAACCGC ACACAAAATA CCTTATCATA TGTTACGTTT CAGTTTATGA CCGCAATTTT
     7501 TAATAACTTC GTATAGCATA CATTATACGA AGTTATtttc agGGAGCCGG ATCTGATTAT
     7561 AAAGACGATG ACGATAAGCG TGACTACAAG GACGACGACG ACAAGCGTGA TTACAAGGAT
     7621 GACGATGACA AGAGActagt TCGACCTGCA GACTGGCTGT GTATAAGGGA GCCTGACATT
     7681 TATATTCCCC AGAACATCAG GTTAATGGCG TTTTTGATGT CATTTTCGCG GTGGCTGAGA
     7741 TCAGCCACTT CTTCCCCGAT AACGGAGACC GGCACACTGG CCATATCGGT GGTCATCATG
     7801 CGCCAGCTTT CATCCCCGAT ATGCACCACC GGGTAAAGTT CACGGGGGAC TTTATCTGAC
     7861 AGCAGACGTG CACTGGCCAG GGGGATCACC ATCCGTCGCC CGGGCGTGTC AATAATATCA
     7921 CTCTGTACAT CCACAAACAG ACGATAACGG CTCTCTCTTT TATAGGTGTA AACCTTAAAC
     7981 TGCATTTCAC CAGCCCCTGT TCTCGTCGGC AAAAGAGCCG TTCATTTCAA TAAACCGGGC
     8041 GACCTCAGCC ATCCCTTCCT GATTTTCCGC TTTCCAGCGT TCGGCACGCA GACGACGGGC
     8101 TTCATTCTGC ATGGTTGTGC TTACCGAACC GGAGATATTG ACATCATATA TGCCTTGAGC
     8161 AACTGATAGC TGTCGCTGTC AACTGTCACT GTAATACGCT GCTTCATAGC ATACCTCTTT
     8221 TTGACATACT TCGGGTtcct aggaaatcga taacatggtc atagctgttt cctgtgtgaa
     8281 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     8341 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     8401 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     8461 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     8521 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     8581 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     8641 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     8701 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     8761 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     8821 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     8881 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     8941 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     9001 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     9061 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     9121 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     9181 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     9241 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     9301 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     9361 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     9421 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     9481 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     9541 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     9601 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     9661 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     9721 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     9781 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     9841 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     9901 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     9961 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    10021 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    10081 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    10141 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    10201 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    10261 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    10321 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    10381 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
    10441 taacctataa aaataggcgt atcacgaggc cctttcgtc  
//
